Bioline JetSeq ER and Ligation Kit Product Manual

This document is a manual for JetSeq™ ER and Ligation Kit


Bioline JetSeq ER and Ligation Kit Product Manual - cover page
Brand
Bioline
Document type
User Manual
Language
English
Pages
14
File format
PDF
File size
271 KB
Published
15 October, 2023
Updated
15 October, 2023
MD5 checksum
6955D83A1E3C830639F28338269EE5A6

About this manual

The Bioline JetSeq ER and Ligation Kit is designed to generate high-quality next-generation sequencing (NGS) libraries suitable for sequencing on Illumina instruments. The kit contains all necessary enzymes and buffers for end-repair, A-tailing, and ligation in convenient master mix formulations, offering a streamlined workflow that can produce adapter-ligated libraries in under 2 hours.

What's inside

  • Kit contents
  • Description
  • Storage
  • Safety information
  • Product specifications
  • Equipment and reagents to be supplied by user
  • Important notices
  • Protocol
  • Library analysis
  • Technical support and troubleshooting

Specifications

Compatible platformsIllumina sequencing platforms
Indicated usewhole-genome sequencing libraries preparation
Quality standard13485 Quality Management System

Frequently asked questions

What is the recommended storage temperature for the kit components?

The kit components should be stored at -20 °C.

How long does it take to produce adapter-ligated libraries with this kit?

Adapter-ligated libraries can be produced in under 2 hours.

What equipment is required for library quality analysis?

A Bioanalyzer, Tapestation, or similar equipment is recommended to check library quality.

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