This manual provides instructions for using Agilent PfuTurbo Hotstart DNA Polymerase, covering materials, storage, optimization parameters, and PCR protocols.
The document serves as an instruction manual for the Agilent PfuTurbo Hotstart DNA Polymerase (Catalog #600320, #600322, #600324). It details the materials provided with each kit, including enzyme units and reaction buffer, along with specific storage conditions of -20°C. The manual outlines notices regarding research use only licensing and lists additional materials required for PCR reactions.
| Enzyme Activity | 2.5 U/μl |
|---|---|
| Storage Temperature | -20°C |
| Recommended Extension Time (<10 kb) | 1 min per kb |
| Recommended Enzyme Concentration (50 μl, <6 kb genomic) | 2.5 U |
Use an extension time of 1 minute per kb.
Typically use amounts of 50–100 ng of DNA template per 50-μl reaction.
Use primers at a final concentration of 0.2–0.5 μM.