A quick reference guide providing optimized protocols for using Agilent's Brilliant III Ultra-Fast SYBR Green QPCR Master Mix with Mx3000P and Mx3005P systems.
This document serves as a quick reference guide for the preparation of reactions using Agilent's Brilliant III Ultra-Fast SYBR Green QPCR Master Mix. It details the steps to dilute the reference dye, combine reagent components including primers and DNA templates, and mix the final reaction mixture without creating bubbles.
| Final reaction volume | 20 μl |
|---|---|
| Reference dye dilution ratio | 1:500 |
| Upstream primer concentration range | 200–500 nM |
| Downstream primer concentration range | 200–500 nM |
It is designed for use with the Mx3000P and Mx3005P QPCR Systems.
The suggested quantity range is 5 pg to 50 ng per reaction.