This user guide provides instructions for using Affymetrix GSEQ 4.1 to generate, evaluate, and export high-quality sequence data from CustomSeq arrays.
The Affymetrix GeneChip Sequence Analysis Software (GSEQ) Version 4.1 is a tool designed to allow scientists to perform comparative sequencing tasks with high accuracy. Key benefits include automated base calling with over 99.9 percent accuracy, reporting of call rates by sample and fragment, and the automatic assignment of homozygote positions with editing tracking. The software supports flexible export options, allowing data to be saved in FASTA format as either all sequences or SNPs only.
The software provides automated base calling with greater than 99.9 percent accuracy.
Cell intensity data uses the .cel file extension.