Detailed protocols, equipment lists, maintenance steps, and troubleshooting for the Affymetrix GeneChip Mapping Assay (Rev. 2).
This manual provides step‑by‑step procedures for conducting GeneChip® Mapping experiments, covering sample preparation, restriction digestion, ligation, PCR, fragmentation, labeling, hybridization, washing, staining, and scanning. It also includes a scientific overview of the assay principle and guidance on laboratory setup to avoid contamination.
Additional sections describe weekly and monthly fluidics‑station maintenance, data‑analysis workflows, a comprehensive troubleshooting guide, technical support contacts, and a complete list of required reagents, instruments, and supplier information.