The Affymetrix PrimeView™ Human Gene Expression Array provides whole‑genome coverage with high specificity and reproducibility for 3’ expression analysis.
The PrimeView™ Human Gene Expression Array is a cartridge‑format microarray designed for use with the GeneChip® Scanner 3000 7G series. It contains 49,395 probe sets, covering 48,658 RefSeq probe sets, 419 UniGene probe sets not covered by RefSeq, and 318 other control probe sets. The array emphasizes well‑annotated genes and uses a perfect‑match‑only design.
Performance data show a median coefficient of variation below 10 % and Pearson correlation coefficients greater than 0.990 for replicate hybridizations. The array is supported by Affymetrix premium reagents and software, and ordering information is provided for 10‑array and 30‑array kits.
| Input RNA required | 50–500 ng |
|---|---|
| Sensitivity | ≥1:100,000 |
| Detectable fold change | ≥2.0 fold |
| Dynamic range | ≥3 log |
| Probe length | 25‑mer |
| Total probe sets | 49,395 |
| RefSeq probe sets | 48,658 |
| UniGene probe sets not covered by RefSeq | 419 |
| Other probe sets (controls and other sources) | 318 |
The array requires 50–500 ng of input RNA.
The array can detect targets at a sensitivity of at least 1 : 100,000.
The array contains a total of 49,395 probe sets.
The dynamic range is three log units or greater.