Product leaflet for GMP‑TL730 recombinant human TIGIT protein, detailing composition, purity, storage, and mechanism of action.
The GMP‑TL730 recombinant human TIGIT protein is expressed in human HEK293 cells and supplied as a lyophilized powder in sterile PBS with 6% mannitol (pH 7.4). The protein consists of 351 amino acids, has a predicted molecular weight of about 40 kDa, and shows ≥95% purity by SDS‑PAGE and HPLC analysis.
The lyophilized product can be stored at 4 °C for up to 24 months, while the reconstituted solution should be kept at –20 °C for 6–12 months, avoiding repeated freeze‑thaw cycles. The protein exhibits biological activity of 1 µg/mL (100 µL per well) and binds human CD155 with a linear range of 10–100 ng/mL. This manual is written in Chinese.
| Molecular weight | 40 kDa |
|---|---|
| Purity | ≥95% |
| Endotoxin level | <0.1 EU/µg |
| Biological activity | 1 µg/mL (100 µL per well) |
| Binding linear range | 10–100 ng/mL |
| Amino acid length | 351 residues |
| Shelf life (dry) | 24 months at 4 °C |
| Shelf life (reconstituted) | 6–12 months at –20 °C |
The lyophilized product can be stored at 4 °C for 24 months, and the reconstituted solution can be stored at –20 °C for 6–12 months, avoiding repeated freeze‑thaw cycles.
The protein purity is ≥95%, confirmed by SDS‑PAGE gel and high‑performance liquid chromatography.
The endotoxin level is less than 0.1 EU per microgram of protein.