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- Manuals
- 5
- Languages
- ZH
- Last added
- 2026-10-01
Featured Beijing T&L Biological Technology manuals
Beijing T&L Biological Technology GMP-TL601 CD3/CD28 Antibody Conjugated Magnetic Beads User Manual
This document describes the AMMS® CD3/CD28 antibody‑conjugated magnetic beads (product code GMP‑TL601) designed for the isolation, activation and expansion of human T lymphocytes, supporting applications such as CAR‑T cell production. It outlines bead preparation, washing, cell‑bead mixing ratios, incubation conditions, and downstream culture steps.
Key procedures include bead washing with PBS containing 1% HSA, mixing beads with cells at a 3:1 ratio (or 1:1 for pure T cells), incubation at 4‑25 °C with gentle shaking, and subsequent culture in IL‑2‑supplemented medium at 37 °C, 5% CO₂ for up to 14 days. Storage, endotoxin limits, and safety precautions are also provided. This manual is written in Chinese.
Covers: Product Information · Bead Washing Procedure · Cell Separation and Expansion · Cell Counting and Culture Expansion · Precautions · References
Beijing T&L Biological Technology GMP-TL101 Anti-human CD3 Monoclonal Antibody Product Specification
The anti‑human CD3 monoclonal antibody (GMP‑TL101) is produced in CHO cells, purified by Protein A affinity chromatography, and is a colorless transparent liquid with purity greater than 95% and endotoxin levels below 0.01 EU/µg. It is intended for research use to activate and expand T‑lymphocytes.
For use, a concentration of 500 ng/ml is recommended for soluble‑phase activation, or coating at 5 µg/ml for plate‑bound (coating) methods. The product should be stored at 2‑8 °C, with a shelf life of 24 months; after aliquoting, store at –20 °C for up to 6 months or –80 °C for up to 12 months to avoid repeated freeze‑thaw cycles. This manual is written in Chinese.
Covers: Product Name · Scope of Application · Product Information · Mechanism of Action · Functional Parameters · Usage Instructions
Beijing T&L Biological Technology GMP-TL730 Recombinant Human TIGIT Protein Product Leaflet
The GMP‑TL730 recombinant human TIGIT protein is expressed in human HEK293 cells and supplied as a lyophilized powder in sterile PBS with 6% mannitol (pH 7.4). The protein consists of 351 amino acids, has a predicted molecular weight of about 40 kDa, and shows ≥95% purity by SDS‑PAGE and HPLC analysis.
The lyophilized product can be stored at 4 °C for up to 24 months, while the reconstituted solution should be kept at –20 °C for 6–12 months, avoiding repeated freeze‑thaw cycles. The protein exhibits biological activity of 1 µg/mL (100 µL per well) and binds human CD155 with a linear range of 10–100 ng/mL. This manual is written in Chinese.
Covers: Product Name · Product Number · Expression Host · Molecular Weight · Purity · Endotoxin Level
Beijing T&L Biological Technology GMP-TL642 Recombinant Human Noggin Protein Product Leaflet
This leaflet provides information on the recombinant human Noggin protein (GMP‑TL642) produced by Beijing T&L Biological Technology. The protein is expressed in human HEK293 cells and is supplied as a lyophilized product for research use.
Key specifications include a theoretical molecular weight of 23.9 kDa, purity of ≥95%, endotoxin level ≤0.01 EU/µg, and biological activity with an ED50 of 2.0‑3.0 ng/mL. The product is stored lyophilized at 4 °C for up to 24 months or reconstituted and kept at –20 °C for 6‑12 months, avoiding repeated freeze‑thaw cycles. This manual is written in Chinese.
Covers: Product Information · Expression Host · Synonyms · Protein Sequence · Molecular Weight · Purity
Latest Beijing T&L Biological Technology manuals
Beijing T&L Biological Technology GMP-TL601 CD3/CD28 Antibody Conjugated Magnetic Beads User Manual
User Manual 2 pages · 249 KB · ZHThe manual provides step‑by‑step instructions for using AMMS® CD3/CD28 antibody‑conjugated magnetic beads (GMP‑TL601) to isolate, activate and expand human…
Beijing T&L Biological Technology GMP-TL101 Anti-human CD3 Monoclonal Antibody Product Specification
Product Leaflet 1 pages · 237 KB · ZHThe GMP‑TL101 anti‑human CD3 monoclonal antibody is a CHO‑derived, >95% pure reagent for T‑cell activation, stored at 2‑8 °C for up to 24 months.
Beijing T&L Biological Technology GMP-TL730 Recombinant Human TIGIT Protein Product Leaflet
Product Leaflet 1 pages · 213 KB · ZHProduct leaflet for GMP‑TL730 recombinant human TIGIT protein, detailing composition, purity, storage, and mechanism of action.
Beijing T&L Biological Technology GMP-TL642 Recombinant Human Noggin Protein Product Leaflet
Product Leaflet 1 pages · 225 KB · ZHProduct leaflet for recombinant human Noggin protein (GMP‑TL642) from Beijing T&L Biological Technology, detailing expression host, purity, molecular…
Beijing T&L Biological Technology GMP-TL645 Recombinant Human TGF-β2 Protein Product Information Sheet
Product Leaflet 1 pages · 225 KB · ZHThe product sheet provides details on the recombinant human TGF‑β2 protein GMP‑TL645, including expression host, purity, molecular weight, activity and…
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User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in Beijing T&L Biological Technology manuals
What bead‑to‑cell ratio should be used for mixed PBMCs?
Mix the magnetic beads with cells at a 3:1 ratio (beads:cells).
What ratio is recommended for pure T‑cell activation?
For pure T cells, adjust the bead‑to‑cell ratio to 1:1.
What is the recommended concentration for using the anti‑human CD3 antibody?
The recommended concentration is 500 ng/ml for soluble‑phase activation.
How should the antibody be stored?
Store the product at 2‑8 °C for up to 24 months; after aliquoting, keep at –20 °C for 6 months or –80 °C for 12 months to avoid freeze‑thaw cycles.
What are the recommended storage conditions for the GMP‑TL730 protein?
The lyophilized product can be stored at 4 °C for 24 months, and the reconstituted solution can be stored at –20 °C for 6–12 months, avoiding repeated freeze‑thaw cycles.
From: Beijing T&L Biological Technology GMP-TL730 Recombinant Human TIGIT Protein Product Leaflet
What is the purity of the recombinant TIGIT protein?
The protein purity is ≥95%, confirmed by SDS‑PAGE gel and high‑performance liquid chromatography.
From: Beijing T&L Biological Technology GMP-TL730 Recombinant Human TIGIT Protein Product Leaflet