The GMP‑TL101 anti‑human CD3 monoclonal antibody is a CHO‑derived, >95% pure reagent for T‑cell activation, stored at 2‑8 °C for up to 24 months.
The anti‑human CD3 monoclonal antibody (GMP‑TL101) is produced in CHO cells, purified by Protein A affinity chromatography, and is a colorless transparent liquid with purity greater than 95% and endotoxin levels below 0.01 EU/µg. It is intended for research use to activate and expand T‑lymphocytes.
For use, a concentration of 500 ng/ml is recommended for soluble‑phase activation, or coating at 5 µg/ml for plate‑bound (coating) methods. The product should be stored at 2‑8 °C, with a shelf life of 24 months; after aliquoting, store at –20 °C for up to 6 months or –80 °C for up to 12 months to avoid repeated freeze‑thaw cycles. This manual is written in Chinese.
| Expression host | CHO cells |
|---|---|
| Purity | >95% |
| Endotoxin level | <0.01 EU/µg |
| Purification method | Protein A affinity chromatography |
| Appearance | Colorless transparent liquid |
| Recommended concentration (soluble method) | 500 ng/ml |
| Storage temperature | 2‑8 °C |
| Shelf life | 24 months |
The recommended concentration is 500 ng/ml for soluble‑phase activation.
Store the product at 2‑8 °C for up to 24 months; after aliquoting, keep at –20 °C for 6 months or –80 °C for 12 months to avoid freeze‑thaw cycles.
The antibody has a purity greater than 95% as determined by SDS‑PAGE and HPLC analysis.