Instruction manual for RayBio® Mouse RANTES IQELISA Kit
This document provides step-by-step instructions for using the RayBio® Mouse RANTES IQELISA Kit, which combines ELISA specificity with qPCR sensitivity. It includes sections on reagent preparation, assay procedure, data calculation, and troubleshooting. The kit uses a 96-well PCR plate and requires a real-time PCR instrument for data collection. Results are calculated using Ct values and standard curves.
| Minimum Quantifiable Dose | 0.48 pg/ml |
|---|---|
| Recovery Rate | 97% |
| Inter-Plate CV | Below 15% |
The minimum quantifiable dose is typically 0.48 pg/ml, though levels below this may be detected outside the quantification range.
The recovery rate is 97%, with a range of 87% to 112%.
The inter-plate CV is below 15%.