RayBiotech Mouse Activin A IQELISA Kit User Manual

This is a mouse Activin A IQELISA kit that uses immuno enzyme linked immunosorbent assay (IQELISA) technology, which has high sensitivity and specificity, and can be used for quantitative measurement of mouse Activin A in serum, plasma, and cell culture supernatants.


RayBiotech Mouse Activin A IQELISA Kit User Manual - cover page
Brand
RayBiotech
Category
Other
Document type
User Manual
Language
English
Pages
11
File format
PDF
File size
153 KB
Published
19 October, 2023
Updated
19 October, 2023
MD5 checksum
18842415DC317FDF90B678E0CBAE95B6

About this manual

This document is the user manual for the RayBio® Mouse Activin A IQELISA Kit (Catalog #: IQM-ActivinA). It describes an innovative assay that combines the specificity of an ELISA with the sensitivity of real-time PCR to quantify Mouse Activin A in serum, plasma, and cell culture supernatants. The kit utilizes an antibody-coated 96-well PCR plate and a detection affinity molecule to enable qPCR readout.

What's inside

  • Introduction
  • Reagents
  • Storage
  • Additional Materials Required
  • Reagent Preparation
  • Assay Procedure
  • Calculation of Results
  • Troubleshooting Guide

Specifications

Minimum quantifiable dose0.19 pg/ml
Sample typesserum, plasma, and cell culture supernatants
Storage temperature (Kit)2° to 8°C
Storage temperature (Standards)-20°C or -80°C
Plate type0.2mL, non-skirted 96-well plate

Frequently asked questions

What is the minimum quantifiable dose of Activin A?

The minimum quantifiable dose is typically 0.19 pg/ml.

What are the recommended storage conditions for the kit?

The kit may be stored for up to 6 months at 2° to 8°C from the date of shipment.

What is the recovery rate for serum spike tests?

Serum spike tests show recovery is 97% with a range from 118% to 85%.

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