The RayBio® Mouse TIM1 IQELISA Kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of Mouse TIM1 in serum, plasma, and cell culture supernatants.
This document provides step-by-step instructions for using the RayBio® Mouse TIM1 IQELISA Kit, including reagent preparation, assay procedure, and data calculation. The kit combines ELISA specificity with qPCR sensitivity for quantifying Mouse TIM1 in biological samples. It requires a real-time PCR instrument and standard lab equipment for setup and analysis.
The protocol includes detailed steps for preparing standards and samples, incubating with detection reagents, and running qPCR. Results are calculated using Ct values, with options for log-log graph analysis or linear regression to determine antigen concentration.
| Minimum Quantifiable Dose | 1.95 pg/ml |
|---|---|
| Recovery Rate | 146% (range 138% to 157%) |
| Interplate CV | Below 15% |
The minimum quantifiable dose of TIM1 is typically 1.95 pg/ml, however levels as lower than 1.95 pg/ml may be detected outside of the quantification range.
Serum spike tests show recovery is 146% with a range from 138% to 157%.