This is a guide from Agilent Technologies about the method of selecting columns and the method of separating peptides and proteins using reverse phase HPLC.
This document serves as a selection guide for Agilent ZORBAX HPLC columns designed for the analysis of biomolecules. It covers applications including Reverse Phase (RP), Size Exclusion (SEC), Ion Exchange (IEC), and Hydrophobic Interaction (HIC) chromatography. The guide specifically highlights the ZORBAX product line for its high resolution and reproducibility in separating peptides and proteins. This manual is written in Japanese.
A column with a 300 A pore size should be selected for samples with a molecular weight of 4-5 kD or more.