This article introduces the Poroshell 120 columns from Agilent Technologies, which offer advantages in speed and resolution. By transferring methods from conventional 3.5 or 5 µm columns, time and money can be saved without upgrading the equipment.
This document provides video notes by Agilent scientists Ron Majors and John Henderson regarding the transfer of High-Performance Liquid Chromatography (HPLC) methods. It explains how moving from conventional 3.5 or 5 µm columns to Agilent Poroshell 120 columns can significantly improve analytical speed and resolution while reducing solvent usage. This manual is written in French.
The total particle size is 2.7 μm.
The particles have a solid core of 1.7 μm and an external porous shell of 0.5 μm.