Agilent 6890Plus/5973N Nitrofen Determination Application Note

About this manual

This document describes an analytical method for determining the herbicide Nitrofen in turkey meat using Gas Chromatography/Mass Spectrometry (GC/MS). The application focuses on the advantages of Negative Chemical Ionization (NCI) mode over Electron Impact (EI) mode for detecting analytes with high electron affinity in complex food matrices.

The text details the experimental setup, including the use of Agilent 6890Plus and 5973N MSD instruments with EPC modules and Retention Time Locking (RTL). It outlines the sample preparation procedure according to DFGS 19 and presents results comparing EI and NCI detection modes, highlighting the superior sensitivity and selectivity of NCI for Nitrofen detection. This manual is written in German.

What's inside

• Keywords
• Introduction
• Negative Chemical Ionization
• Pulsed Splitless Injection
• Retention Time Locking
• Experimental Execution
• Sample Preparation
• GC/MSD Analysis
• Instrumentation
• Results

Specifications

Frequently asked questions

Which detection mode is preferred for Nitrofen?

Negative Chemical Ionization (NCI) mode is preferred because Nitrofen shows high electron affinity.

What is the detection limit for Nitrofen in a turkey meat matrix under the specified NCI SIM conditions?

The detection limit for Nitrofen in the turkey meat matrix is 200 fg.

What is the retention time deviation of the Nitrofen analyte in the spectrum library compared to the matrix sample?

The retention time deviation is +6 seconds.

What is the concentration range used to create the calibration function for Nitrofen SIM measurements?

The calibration function was created in a concentration range from 50 fg/µL to 100 pg/µL.