Pursuit™ UPS 2.4 is an ultra-high resolution, high sensitivity HPLC column that can be run on a standard HPLC system without the need for high pressure. Compared to 3 μm particles, Pursuit UPS 2.4 offers higher resolution and sensitivity, and has lower resistance.
This document describes the Varian Pursuit UPS 2.4 (Ultra Performance Silica) HPLC columns. These columns are designed to provide extreme resolution and sensitivity in standard HPLC applications without requiring very high pressure systems, offering approximately 50% lower backpressure than sub-2 µm columns for nearly equal efficiency.
The leaflet details the column's advantages, including excellent resolution for demanding applications, increased sensitivity compared to 3 µm columns, and reliable performance with sharp peaks. It provides examples of high-resolution separations on standard instruments like the Varian 920-LC and demonstrates trace detection capabilities in LC/MS applications such as herbicide analysis in water and metabolite detection in saliva. This manual is written in French.
The column uses optimized particles that are 2.4 micrometers in size.
The stated flow rates are 200 microliters per minute and 250 microliters per minute.
The temperature condition for that specific test is set to 60 degrees Celsius.