This document is about the instructions for using Agilent Oligo Microarrays, which are for research use only and not for use in diagnostic procedures. The Wash Buffer Additive is a validated automation solution for hybridization and washing of 60-mer oligo microarrays for array-based comparative genome hybridization (aCGH) analysis. It provides increased uniformity when drying under high-pressure nitrogen.
The Wash Buffer Additive is designed to supply a validated automation solution for hybridization and washing of 60-mer oligo microarrays used in array-based comparative genome hybridization (aCGH) analysis. It serves a dual purpose when spiked into the Oligo CGH Wash Buffer 2: providing increased uniformity during drying under high-pressure nitrogen and acting as a cleaning agent for the instrument.
The product is intended for use with Tecan HS Pro instruments when used with Agilent 1X and 2X array formats. It should be stored at room temperature upon receipt and can be kept for up to 12 months after preparation.
It supplies a validated automation solution for hybridization and washing of 60-mer oligo microarrays for array-based comparative genome hybridization analysis.
The product is sufficient for approximately 120 slides, depending on format and use.
For hybridization, spike at 0.01%; for cleaning and conditioning, spike at 0.1%.