This document provides information about the usage and maintenance of Agilent Hi-Plex Ligand Exchange columns. High purity and degassed mobile phases are required. Mobile phases with up to 5% alcohol or 30% acetonitrile can be used. Sample preparation involves filtration and removal of protein, lipid, and other biological contaminants. Regular column efficiency testing is recommended.
This document provides technical specifications and operational guidelines for Agilent Hi-Plex ligand exchange chromatography columns. It covers installation instructions, including the use of industry-standard end fittings and PEEK fittings to prevent dead volume. The guide details column conditioning protocols, emphasizing the need to run at elevated temperatures with a low flow rate before increasing to operating levels.
| Max Pressure | 50 bar |
|---|---|
| Warranty Period | 60 days |
Nuts should be tightened finger tight and then 1/4 turn using a wrench only on the outer column end fitting.
Yes, they may be stored at 4 °C but under no circumstances should they be allowed to freeze.
Up to 5% v/v alcohol can be used in the mobile phase.