The document provides spectral data for LC and LC/MS applications, including retention times for 10 sugars.
This document serves as an essential catalog for Agilent Technologies' Liquid Chromatography (LC) and LC/MS applications. It provides detailed examples of separation methods across various industries, including pharmaceuticals, biopharmaceuticals, and general chemistry.
The content features specific application notes detailing column types, mobile phases, flow rates, temperatures, and detector settings for analyzing compounds such as oligosaccharides, antibodies, amino acids, nucleosides, and barbiturates. It highlights the use of columns like Hi-Plex H, Bio MAb, ZORBAX GF-250, and Poroshell 300SB-C18 to resolve complex mixtures.
| Column Type for Oligosaccharide Separation | Hi-Plex H |
|---|---|
| Flow Rate for Antibody Separation | 0.94 mL/min |
| Column Type for Large Molecules | Poroshell 300SB-C18 and 300SB-C8 |
| Column Type for Nucleosides | ZORBAX SB-C3 |
You can visit the online Chromatogram Library at www.agilent.com/chem/library.
The method uses 10 mM NaHPO4 and 10 mM Na2B4O7 with OPA/FMOC derivatization.