The document describes the features and characteristics of the Agilent Multiple Affinity Removal System. This system allows for the simultaneous removal of multiple high-abundance proteins, enabling easier detection of low-abundance proteins and peptides. It has a larger loading capacity and reusable columns, minimizing sample loss and improving the detection limits of protein separation methods. Additionally, the system minimizes non-specific protein binding without sacrificing efficiency.
The Agilent Multiple Affinity Removal System is designed to unlock the potential of the human and mouse serum proteome by removing multiple targeted high-abundance proteins simultaneously. This automated sample preparation allows for the detection of lower-abundant proteins and peptides that are often masked by high-concentration interferents like albumin, IgG, and transferrin.
| Removal Efficiency (Human) | >98% to >99% |
|---|---|
| Proteins Removed (Human) | Albumin, IgG, IgA, Transferrin, Haptoglobin, Antitrypsin |
| Proteins Removed (Mouse) | Albumin, IgG, Transferrin |
| Column Reusability | At least 200 injections |
No, human protein removal columns are not recommended for mouse serum and vice versa.
The Hu6-HC provides twice the loading capacity compared to the standard Hu-6 in the same column formats.