The document describes a simplified and automated solution for next-generation sequencing (NGS) sample preparation, including optimized liquid handling and labware management protocols, modular design, open platform compatibility, reproducible data, and higher throughput.
Agilent offers comprehensive automation solutions designed specifically for Next Generation Sequencing (NGS) sample preparation. These systems utilize optimized VWorks scheduling protocols and modular components to automate liquid handling and labware management, significantly reducing manual intervention compared to traditional methods.
The brochure details two primary configurations: Agilent NGS Option A and Option B. Both options deliver higher throughput with less hands-on time, capable of preparing up to 960 libraries in a single week for whole genome sequencing or processing 192 samples over a five-day work week for complex targeted resequencing applications.
| Dispensing Volume Range | 300 nL to 250 uL |
|---|---|
| Pipetting Accuracy (2 uL) | ± 3.3 % accuracy |
| Maximum Samples Processed/Day (Library Prep) | 96 samples |
| Weekly Throughput (Option A/B) | Up to 192 samples per week |
| Compatible Sequencers | Illumina and SOLiD |
Automated liquid handling leads to more consistent yields, higher quality data, and up to five-fold increased laboratory throughput compared to manual processing.
For whole genome sequencing applications, 96 samples can be prepared in less than 4 hours.
The system supports SureSelect XT library preparation and target enrichment reagents, as well as various other commercially available reagents for whole genome or targeted resequencing.