This manual describes the use of the Agilent Low RNA Input Linear Amplification Kit. This kit is designed for quantitative and qualitative analysis of low abundance RNA.
This document provides the optimized protocol for the Agilent Low RNA Input Linear Amplification Kit and its PLUS versions (One-Color and Two-Color). The procedure generates fluorescent complementary RNA (cRNA) suitable for use with Agilent oligonucleotide microarrays. It details workflows for both one-color experiments, requiring 200 to 1000 ng of total RNA input, and two-color experiments, requiring 50 to 5000 ng of total RNA input, or a minimum of 10 ng of PolyA+ RNA.
| One-color total RNA input | 200 to 1000 ng |
|---|---|
| Two-color total RNA input | 50 to 5000 ng |
| PolyA+ RNA minimum input | 10 ng |
| Reactions per kit | 20 reactions |
An experienced user should require approximately 6-10 hours to complete this procedure from start to finish.
Yes, the procedure may be broken down into two steps with a break overnight at step 11 on page 15.
There is routinely at least a 100-fold RNA amplification with use of this kit.