This document provides instructions and optimization conditions for using the Agilent Fluorescent Direct Label Kit, as well as its compatibility with other Agilent Microarray Kits. It also includes recommendations for plant RNA quality.
The Agilent Fluorescent Direct Label Kit generates fluorescent cDNA optimized for use with Agilent's catalog cDNA and Oligo microarrays. The kit facilitates the conversion of RNA isolated from two samples into cyanine 3- or cyanine 5-labeled cDNA using reverse transcriptase and other components.
The product number is G2557A.
It is optimized for use with Agilent cDNA Microarray Kits and Agilent Oligo Microarray Kits.
Each labeling reaction requires either 10 pg of total RNA or 200 ng of poly A+ RNA.