Agilent One Color RNA Spike-In Kit is a RNA Spike-In kit produced by Agilent Technologies. It contains 10 positive control transcripts that are optimized to anneal to complementary probes on the microarray with minimal self-hybridization or cross-hybridization.
The Agilent One Color RNA Spike-In Kit is designed as a set of 10 positive control transcripts derived from the Adenovirus E1A gene. These in vitro synthesized, polyadenylated transcripts anneal to complementary probes on Agilent microarrays with minimal cross-hybridization, allowing users to monitor sample amplification, labeling, and microarray processing independently of starting RNA quality.
The kit includes a concentrated Spike-Mix stock and Dilution Buffer. The protocol details the preparation of serial dilutions based on total RNA input amounts (ranging from 200 ng to 1000 ng) and describes how to spike these controls into samples prior to labeling. Results are analyzed using Agilent Feature Extraction software v8.5, which generates a QC Report detailing linear dynamic range, detection limits, and reproducibility via %CV calculations.
Store the kit at -70°C to -80°C in a nondefrosting freezer for up to 1 year from the date of receipt.
Data can be analyzed with Agilent Feature Extraction version 8.5 software, which generates a QC Report.