Lambda ZAP II Undigested Vector Kit INSTRUCTION MANUAL
The document is an instruction manual for the Lambda ZAP II Undigested Vector Kit (Catalog #236201), manufactured by Agilent Technologies. It provides information on materials provided, such as undigested DNA, host strains (XL1-Blue MRF' and SOLR), and helper phages.
The guide covers essential procedures for using the kit, including establishing bacterial stocks, preparing and digesting the vector, ligating inserts, determining background via blue-white selection, titering libraries, and performing plaque lifts. It also includes protocols for hybridizing and screening libraries, as well as in vivo excision of the pBluescript phagemid.
| Undigested Lambda ZAP II DNA Quantity | 20 ug |
|---|---|
| Vector Cloning Sites | Sac I, Not I, Xba 1, Spe 1, EcoR I, and Xho 1 |
| Insert Size Range | 0 to 10 kb |
The Lambda ZAP II vector should be stored at -20°C.
The SOLR strain should be used for plating excised phagemids.
Helper phages should be stored at -80°C.