This manual introduces the use of Agilent PCR-Script Cam Cloning Kit
The Stratagene PCR-Script Cam cloning kit is a PCR cloning method designed to perform in one hour without adding bases to primers. It features a chloramphenicol-resistance gene in the vector to prevent recovery of parental vectors when subcloning from ampicillin-resistant plasmids, ensuring high efficiency and low false positive rates.
The kit contains enough reagents for 25 reactions.
The Control PCR insert has a length of 994 bp.
The ultracompetent cells must be stored at –80°C immediately upon receipt.