AffinityScript Multiple Temperature Reverse Transcriptase is a genetically engineered version of MMLV reverse transcriptase that exhibits improved specific activity over a broad range of cDNA synthesis temperatures from 42°C to 55°C. In addition to being more versatile compared to other reverse transcriptases, AffinityScript reverse transcriptase produces higher cDNA yields and provides superior performance in RT-PCR.
This document is a product catalog entry for the Agilent AffinityScript Multiple Temperature Reverse Transcriptase, catalog number 600109. It describes the enzyme as a genetically engineered version of MMLV reverse transcriptase designed for improved specific activity and higher cDNA yields across temperatures from 42°C to 55°C.
The enzyme performs optimally over the full range of 42-55°C.
The final reaction volume must be 20 ul.
Incubate the reaction at 42-55°C for 1 hour.