This document is the instruction manual for Stratagene QPCR Human Reference Total RNA
The Stratagene QPCR Human Reference Total RNA is composed of total RNA from 10 human cell lines, optimized to maximize representation of gene transcripts present in low, medium, and high abundance. It is carefully screened for contaminating genomic DNA and serves as a generic template ideal for optimizing QRT-PCR assays without depleting precious experimental samples.
The document details applications for assay optimization and standardization across various detection chemistries, including SYBR Green I dye, TaqMan probes, and Molecular Beacons. It includes specific protocols for preparing reactions, RT-PCR programs, generating standard curves, and troubleshooting common issues.
| Quantity per Catalog #750500 | 25 μg (1 μg/μl in 0.1 mM EDTA/RNase-free H2O) |
|---|---|
| Storage Temperature | -80°C |
It serves as a high-quality control for quantitative PCR gene-expression analysis.
No, it is intended for research use only and not for use in diagnostic procedures.