pCMV-Tag 1 is an expression vector for mammalian epitope tagging.
The pCMV-Tag 1 is an epitope tagging vector designed for gene expression in mammalian cells. It allows a target gene inserted into the vector to be tagged with the FLAG epitope (N-terminal, C-terminal, or internal), the c-myc epitope (C-terminal), or both. The resulting constructs can be transfected into mammalian cells and characterized using commercially available tag-specific antibodies.
The kit includes the pCMV-Tag 1 mammalian expression vector, a control vector, and XL1-Blue host strain. Sufficient reagents are provided for 25 reactions and one control reaction. The manual details storage conditions, required additional materials such as T4 DNA ligase and Tag DNA polymerase, and step-by-step procedures for preparing host strains, ligating inserts, transforming bacteria, and verifying the insert.
| Vector Concentration | 1.0 pg/pl |
|---|---|
| Control Vector Quantity | 10 wg |
| Host Strain Volume Provided | 500 ul |
| Reactions Supported | 25 reactions and 1 control reaction |
Users must have T4 DNA ligase, Tag DNA polymerase, Tag DNA polymerase buffer, and TE buffer.
The XL1-Blue Host Strain must be stored immediately at -80°C.
The product is licensed for research use only and not for diagnostic procedures.