The SignalScout Phosphatase Profiling System is a tool for phosphatase analysis, intended for research use only and not for use in diagnostic procedures. The system provides detection and analysis of phosphatases, with high reliability and accuracy.
The Stratagene SignalScout phosphatase profiling system enables researchers to specifically study dephosphorylation by protein tyrosine phosphatases (PTPs). The system includes tagged human phosphatase expression vectors, matching tagged substrate trapping mutant expression vectors, and purified GST-tagged phosphatases available as separate components. Complete kits are available for select phosphatases and include the anti-c-myc antibody for detection of the c-myc-tagged phosphatase expression product.
The manual details the materials provided in System I and II, storage conditions for vectors and enzymes, and additional materials required such as mammalian expression cells, plates, fluorescence plate readers, and specific reagents like DiFMU/DiFMUP or Malachite Green. It outlines protocols for transfecting vectors into mammalian cells, cell lysis, immunoprecipitation using Anti-c-Myc Monoclonal Antibody, and performing pNPP, DiFMUP, and Malachite Green assays to measure phosphatase activity.
| Human Phosphatase Vector Concentration (System I) | 1 ug/ul |
|---|---|
| Substrate Trapping Mutant Human Phosphatase Vector Concentration (System I) | 1 pg/ul |
| Anti-c-Myc Monoclonal Antibody Concentration | 250 ug/ml |
| pNPP Assay Measurement Wavelength | 405 nm |
| DiFMUP Assay Excitation Wavelength | 358 nm |
| DiFMUP Assay Emission Wavelength | 455 nm |
| Malachite Green Assay Measurement Range | 620-660 nm |
All vectors should be stored at -20°C.
The antibody may be stored at 4°C for up to one month.
The catalog numbers are 257260 and 257261.