HaloPlex Target Enrichment System is a target enrichment system for Illumina sequencing.
This guide describes an optimized protocol for using the Agilent HaloPlex target enrichment system to prepare sequencing library samples for Illumina paired-end multiplexed sequencing platforms. It details a 10-step workflow, including genomic DNA digestion, hybridization to HaloPlex probes for target enrichment and sample indexing, capture of target DNA, ligation of fragments, PCR amplification, purification, and validation.
| Compatible Kits | G9901A/B/C, G9911A/B/C, G9921A/B/C, G9903A/B |
|---|---|
| Recommended DNA Input | 200 ng genomic DNA (split among 8 reactions) plus 25 ng excess DNA |
| Total Genomic DNA Amount | 225 ng |
No, the protocol is not compatible with earlier versions of HaloPlex reagent kits, including Agilent part numbers G9900A, G9900B, and G9900C.
A fluorometry-based DNA quantitation method, such as PicoGreen stain or Qubit fluorometry.
Samples should be stored at –20°C.