Agilent Brilliant II QPCR Master Mix Instruction Manual

This document describes Agilent's Brilliant II QPCR Master Mix product, which is intended for research use only and not for diagnostic procedures. The document provides ordering information and technical service contact details, as well as limitations on product warranty.


Agilent Brilliant II QPCR Master Mix Instruction Manual - cover page
Brand
Agilent
Document type
User Manual
Language
English
Pages
22
File format
PDF
File size
202 KB
Published
06 June, 2012
Updated
28 September, 2023
MD5 checksum
0023D1A74C08F11786D40CAAFAAA6503

About this manual

This document is an instruction manual for the Agilent Technologies Brilliant II QPCR Master Mix (Catalog #600804, #600815). It provides detailed information on materials provided, storage conditions, and additional reagents required for quantitative PCR applications. The kit supports both molecular beacon and TaqMan probe technologies.

What's inside

  • Materials Provided
  • Storage Conditions
  • Additional Materials Required
  • Molecular Beacons
  • TaqMan Probes (Hydrolysis Probes)
  • Endpoint vs. Real-Time Measurements
  • PreProtocol Considerations
  • Preparing the Reactions
  • PCR Cycling Program
  • Troubleshooting

Specifications

Reference Dye Concentration1 mM
Master Mix Volume (per kit)2x 2.5 ml

Frequently asked questions

What instruments is the Brilliant II QPCR Master Mix optimized for?

It is optimized for Stratagene Mx3000P, Mx3005P, and Mx4000 systems, as well as the ABI 7900HT real-time PCR instrument.

How should the reference dye be stored?

The reference dye is light sensitive and should be kept away from light whenever possible.

What are the storage conditions for the components?

Store all components at -20°C. Store the 2x master mix at 4°C after thawing.

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