This document describes the Agilent Technologies SureSelectXT Methyl-Seq Target Enrichment System for Illumina Multiplexed Sequencing Protocol Version A.1, which is manufactured with Agilent SurePrint Technology and is for Research Use Only.
This document provides a detailed protocol for using the Agilent SureSelect XT Methyl-Seq Target Enrichment System. It outlines an optimized workflow for preparing bisulfite-sequencing samples compatible with the Illumina paired-end multiplexed sequencing platform.
The guide covers essential pre-experiment requirements, including safety notes and lists of necessary reagents and equipment such as the SureCycler 8800 Thermal Cycler and Agilent 2100 Bioanalyzer. The core protocol details steps for sample preparation (shearing, repair, ligation), hybridization with the SureSelect XT Human Methyl-Seq Capture Library, bisulfite conversion to differentiate methylated segments, indexing, and final pooling for sequencing.
| Research Use Only Status | Not for use in Diagnostic Procedures |
|---|---|
| Compatible Sequencing Platform | Illumina paired-end multiplexed sequencing platform |
No, the SureSelect XT Methyl-Seq Target Enrichment System is for research use only and not for use in diagnostic procedures.
The SureCycler 8800 Thermal Cycler with a 96 well plate module is the required equipment.