This kit can be used to quantitatively measure BAX content in rat tissue homogenates or other related biological fluids.
This manual describes the USCN BAX ELISA kit, which uses a double‑antibody sandwich ELISA to measure BAX protein in rat tissues or other biological fluids. It lists all supplied reagents, required equipment, storage conditions, and the experimental principle of the assay.
Detailed procedures cover sample collection, reagent preparation, plate loading, incubation steps, washing, substrate development, and data calculation, along with warnings and troubleshooting tips. This manual is written in Chinese.
| Detection range | 0.312 ng/mL – 20 ng/mL |
|---|---|
| Limit of detection | 0.105 ng/mL |
| Plate storage temperature | -20 °C |
| Incubation time for detection solution B | 30 minutes at 37 °C |
The kit can detect BAX concentrations from 0.312 ng/mL up to 20 ng/mL.