This document introduces the ELISA Kit product from USCN Life Science Inc. The product is an enzyme-linked immunosorbent assay (ELISA) kit for quantitatively measuring the level of Interleukin 15 (IL15) in mouse serum. The kit utilizes a sandwich ELISA method with dual antibodies, providing high sensitivity and accuracy. It is intended for in vitro research use.
The Uscn E90061Mu 96T ELISA kit is designed for quantitative measurement of mouse IL‑15 in serum, plasma or other biological fluids using a double‑antibody sandwich ELISA. The kit supplies pre‑coated 96‑well plates, standards, detection reagents, TMB substrate and a detailed protocol.
The document describes sample collection, reagent preparation, step‑by‑step incubation times (e.g., 2 h for detection A, 1 h for detection B, 15‑25 min for substrate), washing procedures, data analysis, and safety warnings. Storage conditions for reagents and recommended equipment are also included. This manual is written in Chinese.
| Detection range | 15.6 pg/mL – 1,000 pg/mL |
|---|---|
| Lowest detection limit | 6.4 pg/mL |
| Standard storage temperature | -20 °C (standard) / -80 °C (samples) |
The assay can detect IL‑15 concentrations from 15.6 pg/mL up to 1,000 pg/mL.
Store the TMB substrate protected from light and avoid direct strong illumination during storage and incubation.