Instruction manual for Uscn E0196Fi ELISA kit to quantify fish β‑galactosidase, covering reagents, sample handling, assay steps and storage.
The Uscn E0196Fi ELISA kit provides a double‑antibody sandwich ELISA for quantitative measurement of β‑galactosidase in fish serum, plasma or other biological fluids. The kit includes a pre‑coated 96‑well plate, standards, detection reagents, TMB substrate, stop solution and washing buffer.
The assay principle involves binding β‑GAL to immobilised antibodies, adding biotinylated detection antibody, HRP‑streptavidin, and TMB substrate for colour development measured at 450 nm. The manual details sample collection, reagent preparation, step‑by‑step incubation, washing, colour development, data analysis, storage conditions, and safety warnings. This manual is written in Chinese.
| Plate format | 96‑well |
|---|---|
| Detection range | 0.78 U/L – 50 U/L |
| Limit of detection | 0.195 U/L |
| Recommended storage temperature | -20 °C |
| Required microplate reader filter | 450 ± 10 nm |
A microplate reader with a 450 ± 10 nm filter, single‑ or multi‑channel pipettes, EP tubes, distilled water and absorbent paper are required.
The assay can measure β‑GAL concentrations from 0.78 U/L to 50 U/L.