Agilent GeneSpring Workgroup 5.3 Web Application User Guide is an user manual of Agilent GeneSpring Workgroup 5.3 Web Application, which is a gene expression data mining analysis software released by Agilent Technologies. The software is based on Web browser, and supports non-expert users to perform data mining and analysis on gene expression data, including differentially expressed gene filtering based on p-value and fold-change, principle component analysis, heatmap visualization and so on.