Industrial

The paper introduces SemiQuant method, which is used for preliminary identification of unknown compounds based on mass spectral features, retention time and chemical criteria such as sample extraction and processing. The method can be used to estimate the concentrations of various similar compounds.

Agilent's non-stick O-rings are used to improve GC inlet maintenance, using non-stick O-rings can improve performance and reduce maintenance time

Choosing the right injection port septum for your GC is not as simple as picking up any supplier’s catalog, looking at the temperature range stated for the septum, and assuming that it is made to the same specifications as any other septum. While it is true that there are a limited number of manufacturers of GC injection port septa, the specifications that are applied to one retail supplier are more likely not going to meet the same specifications of another. It would be true to say that only the design engineers of the specific GC’s injection port will know what specifications are needed for the best operation of the inlet as its intended design. Among other criteria, the septum must be of the correct dimensions and elastic density to cause sealing of the injection port in order to allow high-pressure carrier gas to be channeled to the GC column without allowing leaks. It must also do this in such a way to allow a relatively fragile syringe needle to pierce the disk in order to inject a sample into the inlet liner. While the disk is pierced with the needle, it must maintain a seal around the needle until it is eventually removed from the septum, and then the septum must be pliable enough to seal itself off to prevent the vaporous sample from escaping out through the needle’s hole. This self-occluding characteristic is absolutely vital in order to prevent the diffusion of air (containing oxygen) into the inlet. Allowing air into the carrier gas through insid

This document describes the design characteristics of the ferrules used in GC instruments and their contribution to chromatographic signal noise. It also mentions the impact of residue on the background signal during column installation.

This document describes how to build and edit RTL Screener databases, RTL Quant databases, and Mass Spectral Libraries using the GC/MSD ChemStation software and Microsoft Excel.

This document describes the performance of the Agilent 1200 Series variable wavelength detector SL, including the use of different data rates and different detector cells.

The GC PAL and Combi PAL from CTC Analytics are flexible and expanded sample introduction tools that can meet the diverse application requirements in laboratories. These samplers offer a wide range of functionalities and customizable processes to accommodate specific situations. They support various liquid injection volumes, injection styles, and also enable automated solid-phase micro-extraction, heated headspace sampling, continuous flow sampling, and other approaches.

This document introduces the performance of the Agilent microarray platform in the MAQC study.

This paper evaluates the reproducibility of microarray technology and presents metrics for evaluating quality and reproducibility. The authors use data from the Agilent microarray system and Rosetta Resolver algorithms to demonstrate that the system produces data with high confidence for gene expression interpretations. The paper also provides metrics for calculating the reproducibility of microarray technology, including metrics for the microarray preparation process, signal statistics for each gene on each array, signal statistics for each gene across microarrays spotted with the same sample, log ratio statistics for all genes on each self-self microarray, log ratio statistics for each gene on each differential expression microarray, log ratio statistics for each gene across all differential expression microarrays, and biological replicate metrics.

This article introduces a new hybridization apparatus and method that improves the hybridization quality, consistency, and ease of use of the existing system. The apparatus improves hybridization quality by providing a hybridization chamber made from inert materials that enable easy hybridization buffer mixing. Consistency is improved through material quality and high-quality manufacturing. Finally, ease-of-use is enhanced through protocols that utilize standard lab equipment and a straightforward hybridization chamber assembly. The apparatus has been validated for use with Agilent's in-situ synthesized oligonucleotide microarrays and exhibits equivalent or superior experimental performance compared to Agilent's current standard hybridization method.

This Technical Note compares the Agilent 1100 Series diode array detector (DAD) “B” model to its successor the Agilent 1200 Series DAD “D model”. For this comparison both detectors were interchangeably connected to an Agilent 1100 Series LC system. To exclude undesired instrument bias the same Agilent 1100 Series LC system was used for both series of experiments. The evaluated performance criteria included retention time, peak width, resolution, peak area, peak height, signal-to-noise, limit of detection, limit of quantitation and linearity. Overall, the Agilent 1200 Series DAD compared favorably to the Agilent 1100 Series DAD, delivering the same or even noticeably better results. Based on these results it was concluded that an Agilent ChemStation method that was developed and validated on an 1100 Series DAD is fully compatible with the 1200 Series DAD. This means that an 1100 Series DAD can be replaced by a 1200 Series DAD with no or minimal revalidation effort.

This document introduces the Agilent 1200 Series Rapid Resolution LC system, which is the ideal instrument for very fast analysis up to 20 times faster than conventional HPLC with the same or better data quality in terms of resolution.

This overview describes a simple approach to cleaning and re-installing the 5973 and 5975 ion electron impact (EI) inert and standard sources and the chemical ionization (CI) source.