PerkinElmer LS50B Luminescence Spectrometer SOP

About this manual

This document serves as a Standard Operating Procedure (SOP) for the PerkinElmer LS50B Luminescence Spectrometer. It details the steps required to turn on the instrument using its toggle button and login to the associated computer running FL-WinLab software.

The guide provides instructions on accessing the sampling tray, configuring scan parameters such as excitation and emission wavelengths, and utilizing real-time options like background spectrum selection. It also covers data processing features for viewing spectral curves and saving results in text or Excel formats.

What's inside

• Turning ON the Instrument
• Running the Instrument
• Sample Tray Access
• Data Acquisition
• Realtime Options
• Processing Data
• Saving Data

Frequently asked questions

What type of cells are required for the LS50B spectrometer?

The instrument requires 1.0 cm path length cells that must be transparent on all four sides.

What are the default parameters for an emission scan?

The default settings include a start of 340, end of 480, emission of 388, Ex Slit of 5, Em Slit of 2.5, and Scan Speed of 50.

How is the background spectrum name stored in the dataset?

The name of the background spectrum is saved in the header of the data set within the "FL MEMO" field.