This manual provides instructions for using the Maxim EliVision DAB Detection Kit (Enhanced Polymer) to visualize mouse and rabbit primary antibodies on…
The Maxim EliVision DAB Detection Kit (Enhanced Polymer) is designed for immunohistochemical staining of target antigens in routinely processed tissue sections. The kit includes reagents such as reaction enhancer, enzyme-conjugated polymer, and stable DAB substrates to produce a yellow or brown-yellow precipitate at antigen sites. This manual is written in Chinese.
| pH Value of Stable DAB Buffer | 7.6±0.2 |
|---|---|
| Storage Temperature | 2-8°C |
| Shelf Life | 12 months |
| Packaging Sizes | 60, 180, or 1100 tests per box |
The reaction enhancer connects the enzyme-labeled polymer to the primary antibody, and the horseradish peroxidase on the polymer catalyzes DAB decomposition to oxidize diaminobenzidine into diaminophenylamine.
A yellow or brown-yellow precipitate appears in the area where the antigen is present.
The freshly prepared DAB staining solution must be stored in the dark and is valid for 24 hours.