This manual describes the storage, composition, usage, and precautions for Inorganic Phosphorus Solid Medium (PVK/PKO) used to screen phosphate-solubilizing…
The document provides instructions for using Inorganic Pikovskaya Medium (also known as PVK or PKO), which is designed to measure the ability of microorganisms to decompose inorganic phosphorus and to screen for phosphate-solubilizing bacteria or fungi. The medium allows for qualitative assessment by observing the formation of a transparent zone around bacterial colonies. This manual is written in Chinese.
Store the medium at room temperature, where it remains effective for 3 years.
Sterilize the medium at 121°C for 15 minutes or at 115°C for 20 minutes.
Adjust the pH of the prepared medium to 7.0 ± 0.2.
Inoculate the strains onto the plates and incubate them upside down in an incubator at 30°C for 3 to 7 days.