This manual provides instructions for using the ExCell Bio Mycoplasma Detection Kit (MB000-1391/1392) to detect mycoplasma contamination in cell cultures…
The ExCell Bio Mycoplasma Detection Kit is designed to detect common mycoplasma contamination in cell cultures, including species such as M. Hyorhinis, M. Arginini, and M. Orale. The kit utilizes a direct PCR method for high sensitivity and is suitable for testing cell lysates or culture supernatants. It includes components for cell lysis and pre-mixed PCR reagents for specific mycoplasma detection. This manual is written in Chinese.
| Catalog Numbers | MB000-1391 (48 rxn), MB000-1392 (96 rxn) |
|---|---|
| Storage Condition | -20℃ |
| Transport Condition | Dry ice transport |
The kit detects common species including M. Hyorhinis, M. Arginini, M. Orale, M. Fermentans, and A. laidlawii, which represent over 98% of cell mycoplasma contamination.
The recommended conditions are: 95°C for 30 sec, 95°C for 30 sec, 56°C for 30 sec for 30 cycles, followed by 72°C for 30 sec and 72°C for 5 min.