This manual provides instructions for using the ExCell Bio TrueLib mRNA Library Prep Kit (NGS00-2011) to prepare mRNA libraries for Illumina NGS platforms.
The ExCell Bio TrueLib mRNA Library Prep Kit for Illumina is designed for constructing mRNA libraries compatible with Illumina high-throughput sequencing platforms. The kit includes reagents for mRNA isolation, fragmentation, cDNA synthesis, and library construction, excluding adapters and PCR primers. It features a streamlined workflow where end repair and dA-tailing are completed in one step without intermediate purification, followed by enrichment using the ExHiFi high-fidelity DNA polymerase for bias-free PCR amplification. The kit is optimized for short construction time, high yield, and low magnetic bead consumption. This manual is written in Chinese.
| Input RNA Amount | 100ng - 1μg total RNA |
|---|---|
| Storage Temperature | -20℃ |
| Shelf Life | 1 year |
The kit is suitable for transcriptome library construction starting with 100ng to 1μg of total RNA.
The kit is suitable for transcriptome library construction using starting templates ranging from 100 ng to 1 μg of total RNA.
The extracted total RNA should have a RIN value of at least 8, as verified by an Agilent 2100 Bioanalyzer.
The library construction process is completed in 6 hours and 30 minutes.