ExCell Bio Manuals

ExCell Bio TrueLib mRNA Library Prep Kit for Illumina User Manual

The ExCell Bio TrueLib mRNA Library Prep Kit for Illumina is designed for constructing mRNA libraries compatible with Illumina high-throughput sequencing platforms. The kit includes reagents for mRNA isolation, fragmentation, cDNA synthesis, and library construction, excluding adapters and PCR primers. It features a streamlined workflow where end repair and dA-tailing are completed in one step without intermediate purification, followed by enrichment using the ExHiFi high-fidelity DNA polymerase for bias-free PCR amplification. The kit is optimized for short construction time, high yield, and low magnetic bead consumption. This manual is written in Chinese.

Covers: Product Overview · Product Components · mRNA Isolation/Fragmentation · Double-stranded cDNA Synthesis · Double-stranded cDNA Purification · End Repair and dA-tailing