User manual for ExCellBio OptiVitro MSC proliferation serum-free medium, covering preparation, culture, passaging, and storage instructions for human…
This document provides instructions for the ExCellBio OptiVitro MSC proliferation serum-free medium (catalog numbers ME000-N023 and ME000-N023S). It is designed for the culture and expansion of purified human mesenchymal stem cells (hMSCs) under serum-free and xeno-free conditions. The medium helps maintain the long-term culture and multiple passages of hMSCs while preserving their differentiation potential into osteoblasts, chondrocytes, and adipocytes. This manual is written in Chinese.
| Product Application | For research use only, not for clinical disease diagnosis |
|---|---|
| Base Medium Storage | 2-8°C, avoid light |
| Additive Storage | -20°C or lower |
| Base Medium Shelf Life | 9 months |
| Additive Shelf Life | 12 months |
| Culture Conditions | 37-38°C, 5% CO2, saturated humidity |
No, this product is for research use only and its safety and efficacy for clinical diagnosis have not been determined.
The additive component must be stored at -20°C or lower, preferably in a non-frost-free freezer.
No, the product does not contain antibiotics, but they can be added if needed.
The prepared medium should be used within 2 weeks when stored at 2-8°C in the dark.