The DaYou mouse TNF-α ELISA kit (DKW12-2720-048 / DKW12-2720-096) manual provides product overview, reagents, protocol, and analysis instructions.
This manual describes the DaYou® mouse TNF‑α ELISA kit, a pre‑coated plate assay for quantitative detection of natural and recombinant TNF‑α in cell culture supernatants, mouse serum, plasma and other fluids. The kit includes pre‑coated plates, biotinylated antibody, streptavidin‑HRP, standards, washing buffer, TMB substrate and stop solution, and is intended for research use only.
The document outlines the required reagents and equipment, detailed step‑by‑step procedures for sample preparation, incubation, washing, detection, and plate reading, as well as troubleshooting tips, storage recommendations, and reference information for related ELISA products. This manual is written in Chinese.
| Detection range | 1000‑15.6 pg/mL |
|---|---|
| Sensitivity | 8 pg/mL |
| Repeatability | CV < 10 % (intra‑ and inter‑plate) |
| Storage temperature | 4 °C, up to 1 month |
Incubate the plate with the biotinylated detection antibody at 37 °C for 90 minutes.
Read the plate at 450 nm (with a reference wavelength of 610‑630 nm for better accuracy).
Keep TMB protected from light; discard it if it turns blue, indicating contamination.
Store all reagents according to the label instructions, typically at 4 °C, and avoid repeated freeze‑thaw cycles.