The Da You 6031011 Freezing Reagent Kit manual provides detailed procedures for cell freezing, thawing, and experimental record keeping.
This manual describes the Da You® Freezing Reagent Kit (Cat# 6031011) designed for research use, especially ELISPOT assays. It lists the kit components, required reagents, and equipment needed for successful cell cryopreservation and recovery.
The document provides step‑by‑step protocols for cell freezing, thawing, and post‑thaw handling, along with critical precautions such as temperature control, DMSO handling, and recommended cooling rates. It also includes a section for experimental records to document results and observations. This manual is written in Chinese.
| Resuspension Medium volume | 25 mL |
|---|---|
| Resuspension Medium storage temperature | -20 ℃ |
| 2× Freezing Medium volume | 25 mL |
| 2× Freezing Medium storage temperature | -20 ℃ |
The protocol recommends adjusting the cell suspension to 2 × 10⁷ cells/mL before adding the freezing medium.
A cooling rate of 1 ℃ per minute is recommended until the samples reach –80 ℃.