Instruction manual for the DaYo Human G‑CSF ELISA kit (DKW12‑1731‑048 / DKW12‑1731‑096) covering reagents, protocol, and analysis.
This manual provides a complete overview of the DaYo Human G‑CSF ELISA kit, including product description, background information on G‑CSF, and a list of reagents supplied with the kit. It also details the additional equipment and consumables the user must prepare before starting the assay.
Step‑by‑step procedures cover reagent preparation, sample and standard addition, incubation times, washing steps, detection, and plate reading. The document ends with guidance on result interpretation, storage conditions, and troubleshooting tips. This manual is written in Chinese.
| Detection range | 4000‑62.5 pg/mL |
|---|---|
| Sensitivity | 30 pg/mL |
| Repeatability (intra‑ and inter‑assay CV) | < 10 % |
| Maximum total incubation time | ≤ 4 hours |
| Plate type | Pre‑coated ELISA plate (8×12 or 8×6 wells) |
The kit can quantify G‑CSF concentrations from 4000 pg/mL down to 62.5 pg/mL.
The assay sensitivity is 30 pg/mL.
The overall incubation time must not exceed 4 hours; typically 2 hours for sample incubation, 1 hour for detection antibody, and 20 minutes for enzyme conjugate.