Key facts
- Manuals
- 2
- Languages
- ZH
- Last added
- 2026-10-01
Featured 达优 manuals
Da You 6031011 Freezing Reagent Kit User Manual
This manual describes the Da You® Freezing Reagent Kit (Cat# 6031011) designed for research use, especially ELISPOT assays. It lists the kit components, required reagents, and equipment needed for successful cell cryopreservation and recovery.
The document provides step‑by‑step protocols for cell freezing, thawing, and post‑thaw handling, along with critical precautions such as temperature control, DMSO handling, and recommended cooling rates. It also includes a section for experimental records to document results and observations. This manual is written in Chinese.
Covers: 产品说明 · 试剂盒内容 · 实验者自备的试剂与仪器 · 细胞冻存程序 · 细胞复苏程序 · 注意事项
DaYou Mouse TNF-α ELISA Kit DKW12-2720-048 / DKW12-2720-096 User Manual
This manual describes the DaYou® mouse TNF‑α ELISA kit, a pre‑coated plate assay for quantitative detection of natural and recombinant TNF‑α in cell culture supernatants, mouse serum, plasma and other fluids. The kit includes pre‑coated plates, biotinylated antibody, streptavidin‑HRP, standards, washing buffer, TMB substrate and stop solution, and is intended for research use only.
The document outlines the required reagents and equipment, detailed step‑by‑step procedures for sample preparation, incubation, washing, detection, and plate reading, as well as troubleshooting tips, storage recommendations, and reference information for related ELISA products. This manual is written in Chinese.
Covers: Product Introduction · Knowledge Background · Reagents Provided · Reagents and Instruments to Prepare · Precautions · Reagent Preparation
Latest 达优 manuals
Da You 6031011 Freezing Reagent Kit User Manual
User Manual 12 pages · 344 KB · ZHThe Da You 6031011 Freezing Reagent Kit manual provides detailed procedures for cell freezing, thawing, and experimental record keeping.
DaYou Mouse TNF-α ELISA Kit DKW12-2720-048 / DKW12-2720-096 User Manual
User Manual 20 pages · 320 KB · ZHThe DaYou mouse TNF-α ELISA kit (DKW12-2720-048 / DKW12-2720-096) manual provides product overview, reagents, protocol, and analysis instructions.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in 达优 manuals
What cell concentration is recommended for freezing?
The protocol recommends adjusting the cell suspension to 2 × 10⁷ cells/mL before adding the freezing medium.
What cooling rate should be used when freezing the cells?
A cooling rate of 1 ℃ per minute is recommended until the samples reach –80 ℃.
How long should the detection antibody be incubated?
Incubate the plate with the biotinylated detection antibody at 37 °C for 90 minutes.
From: DaYou Mouse TNF-α ELISA Kit DKW12-2720-048 / DKW12-2720-096 User Manual
What wavelength is used to read the ELISA plate?
Read the plate at 450 nm (with a reference wavelength of 610‑630 nm for better accuracy).
From: DaYou Mouse TNF-α ELISA Kit DKW12-2720-048 / DKW12-2720-096 User Manual