Instructions for using the Dakewe DKW12-2060-048/096 mouse IL-6 ELISA kit, covering reagent preparation, assay steps, and result analysis.
The Dakewe mouse IL-6 ELISA kit is a pre‑coated, sandwich‑type ELISA designed for quantitative detection of IL‑6 in cell culture supernatants, mouse serum, plasma and other fluids. The kit includes cytokine standard, biotinylated antibody, streptavidin‑HRP, washing buffer, TMB substrate, stop solution, a pre‑coated 96‑well plate and a sealing film.
The assay procedure involves reagent mixing, sample and standard addition, incubation (up to 2.5 h total), multiple washing steps (8 washes), enzyme reaction with TMB, and termination with stop solution before reading absorbance at 450 nm (reference 610‑630 nm). The manual also provides storage conditions, troubleshooting tips, and a list of compatible instruments. This manual is written in Chinese.
| Detection range | 1000‑15.6 pg/mL |
|---|---|
| Sensitivity | 7 pg/mL |
| Repeatability (intra‑ and inter‑plate CV) | < 10 % |
| Maximum incubation time | 2.5 hours |
The kit can quantify IL‑6 concentrations from 15.6 pg/mL up to 1000 pg/mL.
The assay sensitivity is 7 pg/mL.
No, the kit is for research purposes only and not for diagnostic applications.